Detailed view for TGME49_217600

Basic information

TDR Targets ID: 259281
Toxoplasma gondii, calcium-dependent protein kinase CDPK9
Source Database / ID:  ToxoDB 

pI: 7.2403 | Length (AA): 666 | MW (Da): 75601 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF13202   EF hand
PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005509   calcium ion binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
48 540 2acx (A) 39 526 19.00 0 1 0.83 0.06
125 566 2etr (A) 6 403 22.00 0 1 0.46 0.65
599 666 2ami (A) 8 74 34.00 0.0000013 1 0.69 -1.76
12 557 3pvu (A) 30 566 20.00 0 1 0.77392 0.84
172 664 3hx4 (A) 40 501 34.00 0 1 0.98394 0.16
181 331 4aoj (A) 508 670 26.00 0.0019 1 0.585127 -0.58
190 664 4ysj (A) 35 478 40.00 0 1 1.02461 -0.01
211 520 2w4o (A) 63 335 44.00 0 1 0.693865 0.32
291 420 2izr (A) 145 249 34.00 0.57 0.33 0.138595 1.1
564 666 1pva (A) 8 108 25.00 0 1 0.501755 -1.2
598 664 4gjf (A) 12 81 43.00 0.46 1 0.625001 -1.74
600 666 2m97 (A) 2 67 36.00 0 1 0.708701 -2.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_165980)

Species Accession Gene Product
Neospora caninum NCLIV_062380   CAM kinase, CDPK family CAM kinase TgTPK5, putative
Toxoplasma gondii TGME49_217600   calcium-dependent protein kinase CDPK9

Essentiality

TGME49_217600 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_217600 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 21.9% 334 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 27.2% 331 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 30.0% 317 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 28.8% 306 aa Compounds References
Plasmodium falciparum (isolate 3D7) Calcium-dependent protein kinase 4 528 aa 34.2% 500 aa Compounds References
Bos taurus Glycogen synthase kinase-3 beta splice variant X1 419 aa 23.9% 339 aa Compounds References
Sus scrofa Glycogen synthase kinase 3 beta 420 aa 23.9% 339 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 27.0% 178 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 28.8% 306 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 23.2% 328 aa Compounds References
Zea mays CaM kinase I alpha 492 aa 31.4% 487 aa Compounds References
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 27.1% 306 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 25.5% 325 aa Compounds References
Rattus norvegicus Glycogen synthase kinase-3 beta 420 aa 23.9% 339 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 29.7% 317 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 23.0% 331 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 26.5% 343 aa Compounds References
Triticum aestivum Calcium dependent protein kinase 548 aa 31.2% 487 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 22.1% 294 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 24.4% 324 aa Compounds References
Bos taurus Calmodulin 149 aa 36.4% 151 aa Compounds References
Patiria pectinifera Cdc2 300 aa 25.9% 340 aa Compounds References
Sus scrofa Casein kinase I isoform alpha 125 aa 26.4% 125 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0059 1 1
0.0023 0.5 0.5
0.0081 0.5 0.5
0.0007 0.5 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0026 0.5 0.5
0.0022 0.5 0.5
0.0012 0.5 0.5
0.0059 1 1
0.0029 0.5 0.5
0.0064 0.3377 0
0.0098 0.3242 0.2614
0.0063 1 1
0.0037 1 0.5
0.0039 0.5 0.5
0.0056 1 0.5
0.0004 0.5 0.5
0.0061 0.6883 0.6656
0.0062 0.6935 0
0.0011 1 0.5
0.0081 1 0.5
0.0012 0.5 0.5
0.0036 0.5 0.5
0.0016 0.5 0.5
0.0007 0.5 0.5
0.0032 0.5 0.5
0.0066 0.3101 0
0.0092 1 0.5
0.0069 0.3067 1
0.0033 1 1
0.0012 0.5 0.5
0.0033 0.5 0.5
0.0003 0.5 0.5
0.0093 0.8828 0
0.0027 1 0.5
0.0039 0.5 0.5
0.0091 1 0.5
0.0018 0.5 0.5
0.0008 0.5 0.5
0.0032 0.5 0.5
0.0088 0.4477 1
0.0059 1 1
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0063 0.7244 0.7244
0.0039 0.9485 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_217600 (Toxoplasma gondii), calcium-dependent protein kinase CDPK9
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